Mancini immunology kit for 20 pairs
L'immunité adaptative
The Mancini technique or simple radial immunodiffusion test is based on the formation of immune complexes (antigen-antibody specific complex) using a petri dish coated with a constant height agar in which a serum containing anti-antigen antibodies is mixed.
The general interest of the action is to show that one can not only detect but also dose an antigen in a solution. It also makes it possible to approach the concept of standard range and have students build it using a spreadsheet directly from their experimental data.

Aim of the MANCINI test we offer:
The assay consists of assaying a BSA antigen using an agar petri dish containing antibodies specific for this antigen.

This PW therefore takes place in 2 sessions:

• a first session where the petri dishes are poured and the deposits made;
  
• a second session during which the results are read, the standard range is carried out with a spreadsheet, the unknown concentration is determined.
  
  Composition of the kit (12 to 20 pairs of students):
  20 test dishes and 10 training dishes
  
• 30 petri dishes - 5.5 cm diameter
  
• 30 die cutter droppers
  
• 2 g of agarose
  
• 2 tubes of 1 ml of anti-BSA antibodies
  
• 2 tubes of 1ml BSA (QSP for 3 standards, 1 unknown)
  
• 1 tube of 20 ml of PBS 10X
  
• Technical and educational instructions
  Necessary material:
  
• Variable volume micropipette 1-10 μL
  
• 10 μL micropipette cones
  
• 250 ml Erlenmeyer
  
• Microwave or hotplate
  
• Humid chamber
  
• Graduated ruler for measurement