Adaptive immunity

 
Classic kit
Cognitive objectives:
Fundamental aspects of the functioning of the immune system: The example of AIDS. Technique identical to the AIDS test.
Detection and assay of antibodies by the ELISA technique (micro- titrage on Sheet).

Proposed activity:
Assay an anti-BSA rabbit antibody using a BSA-lined support and couple peroxidase with rabbit immunoglobulin rabbit antibody.
We supply you with ready-to-use Sheets. You perform a standard range of dilution of the antibody to be assayed (anti-BSA) as well as some controls including a serum non-immune against BSA. In addition, each pair of students will be able to assay an immune serum of unknown concentration by comparing it to the standard range.

Results obtained:
The higher the concentration of anti-BSA antibody deposited, the more intense the blue colouring.

Composition (for 24 pairs of students):

• 24 snap-off strips of 8 micro titrage cups with flat or round bottom
• 2 strip holders
• PBS-Tween buffer
• PBS x 10
• 110μl of rabbit serum immunised against BSA
• 110μl of non-immunised rabbit serum
• 50μL of anti-rabbit immunoglobulin antibody coupled with
  peroxidase
• 10ml of sterile water
• 10ml of TMB revealing solution
  Technical and educational instructions available on the site.
  
  Necessary material:
  0 to 200μl Micropipettes (Reference MSR200)
  Conservation: 6 months
  Serums and antibodies in the freezer
  The rest in the refrigerator

Réf : ELISA
 
Simplified kit
This kit makes it possible to test the presence or absence of antibodies.
Quick to implement, since there is no need to carry out a standard range to achieve this PW.
The delivered strips are snap-off and make it possible to create 32 positions with 3 wells: a positive control, a negative control, and the solution to be tested.

Composition (for 24 pairs of students):

• 12 snap-off strips of 8 micro titrage cups with flat or round bottom
• 1 strip holder
• PBS-Tween buffer
• PBS x 10
• 50μl of rabbit serum immunised against BSA
• 500μl of non-immune rabbit serum
• 25μL of anti-rabbit immunoglobulin antibody coupled with peroxidase
• 10 ml of sterile water
• 5 ml of TMB revealing solution
  Technical and educational instructions available on the site.

Réf : ELISAECO